Contact an Account ManagerHPLC determination of reproterol in plasma using on-line trace enrichment and amperometric detection with a rotating working electrode
Summary
Type: HPLC
Application #: 104600
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: amperometric, home-made detector with rotating-disc carbon paste working electrode, 0,90V versus Ag/AgCl
Conditions: eluent for trace enrichment: 0.01F aqueous phosphate - citrate buffer pH 7.0, flow rate 1ml/min; optimized eluent for analytical separation: 0.01F phosphate - citrate buffer pH 7.0 - 7.2, 2.2 - 2.6% propanol-1, 5·10-3 to 6·10-3F tetrabutylammonium hydrogen sulphate
flow rate 1.2ml/min
Authors: Hageman RJJ
Source: J Chromatogr 274 (1983) 239 - 253
Details
Legend: Fig. 6. Chromatograms of A) blank plasma and B) plasma spiked with 0.55ng/ml
reproterol·HCl, I = time of injection, R = reproterol, IS = internal standard
D4908.